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human casr expressing plasmid  (Shanghai Genechem Ltd)

 
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    Shanghai Genechem Ltd human casr expressing plasmid
    Human Casr Expressing Plasmid, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+casr+expressing+plasmid/casr+expressing+human+plasmid/pmc12856587-14-1-21
    Average 86 stars, based on 1 article reviews
    human casr expressing plasmid - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: Identification and functional characterization of a novel nonsense mutation of CASR gene in a familial hypocalciuric hypercalcemia pedigree
    Article Snippet: Multimedia component 1 Multimedia component 1 Figure S1 Construction and confirmation of mutant CASR plasmid and its expression. (A) Vector map of CASR W600∗ plasmid tagged with an N-terminal FLAG epitope. .. The human CASR expressing plasmid was generated by inserting a PCR-amplified human CASR cDNA (NM_001178065.1) fragment into vector GV741 (CMV enhancer-MCS-polyA-EF1A-mCherry-sv40-puromycin, Shanghai Genechem Co., Ltd., China) using KpnI and AgeI restriction enzymes, and a premature stop codon (TAG) was introduced at position c.1799 via overlap PCR to get CASR W600∗ plasmid. ..

    Plasmid Preparation:

    Article Title: Identification and functional characterization of a novel nonsense mutation of CASR gene in a familial hypocalciuric hypercalcemia pedigree
    Article Snippet: Multimedia component 1 Multimedia component 1 Figure S1 Construction and confirmation of mutant CASR plasmid and its expression. (A) Vector map of CASR W600∗ plasmid tagged with an N-terminal FLAG epitope. .. The human CASR expressing plasmid was generated by inserting a PCR-amplified human CASR cDNA (NM_001178065.1) fragment into vector GV741 (CMV enhancer-MCS-polyA-EF1A-mCherry-sv40-puromycin, Shanghai Genechem Co., Ltd., China) using KpnI and AgeI restriction enzymes, and a premature stop codon (TAG) was introduced at position c.1799 via overlap PCR to get CASR W600∗ plasmid. ..

    Generated:

    Article Title: Identification and functional characterization of a novel nonsense mutation of CASR gene in a familial hypocalciuric hypercalcemia pedigree
    Article Snippet: Multimedia component 1 Multimedia component 1 Figure S1 Construction and confirmation of mutant CASR plasmid and its expression. (A) Vector map of CASR W600∗ plasmid tagged with an N-terminal FLAG epitope. .. The human CASR expressing plasmid was generated by inserting a PCR-amplified human CASR cDNA (NM_001178065.1) fragment into vector GV741 (CMV enhancer-MCS-polyA-EF1A-mCherry-sv40-puromycin, Shanghai Genechem Co., Ltd., China) using KpnI and AgeI restriction enzymes, and a premature stop codon (TAG) was introduced at position c.1799 via overlap PCR to get CASR W600∗ plasmid. ..

    Polymerase Chain Reaction:

    Article Title: Identification and functional characterization of a novel nonsense mutation of CASR gene in a familial hypocalciuric hypercalcemia pedigree
    Article Snippet: Multimedia component 1 Multimedia component 1 Figure S1 Construction and confirmation of mutant CASR plasmid and its expression. (A) Vector map of CASR W600∗ plasmid tagged with an N-terminal FLAG epitope. .. The human CASR expressing plasmid was generated by inserting a PCR-amplified human CASR cDNA (NM_001178065.1) fragment into vector GV741 (CMV enhancer-MCS-polyA-EF1A-mCherry-sv40-puromycin, Shanghai Genechem Co., Ltd., China) using KpnI and AgeI restriction enzymes, and a premature stop codon (TAG) was introduced at position c.1799 via overlap PCR to get CASR W600∗ plasmid. ..



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